rEK - Recombinant Enterokinase
Recombinant Enterokinase (rEK) is a highly purified enzyme using pichia pastoris expression system. A single glycosylated Polypeptide chain containing 235 amino acids. Our rEK is manufactured in a highly sterile environment using established scientific procedures and high-grade chromatography techniques.
- Pichia pastoris expression system
- Protein purity > 95% by SDS-PAGE
- rEK is a specific protease that cleaves at Asp-Asp-Asp-Asp-Lys in proteins
- One unit cleaves 50μg of an EGF-EK fusion protein in 16hrs at room temperature
- Generally supplied in 20 mM Tris-HCI, 200 mM NaCl, 2mM CaCl2, 50% glycerol (pH 7.4 @ 4°C)
- Store at -20˚C
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About Enterokinase
Enterokinase is an enzyme produced by the cells of the duodenum and assists the digestion process in humans and animals. It is secreted from the intestinal glands upon the entry of ingested food passing from the stomach. Enterokinase, therefore, aids in the activation of pancreatic digestive enzymes specifically turning trypsinogen to trypsin. The deficiency of this enzyme causes severe disturbance to protein digestion and results in protein malabsorption with poor growth and development.
Enterokinase is highly suited for the biotechnological and biochemical exclusion of fusion proteins and cleavage of tags. It is also a universally used enzyme in the nutrition and biopharma industries. However, the challenges associated with the existing enterokinase include low purity, endotoxins, and variations in batches.
